3 Fold Serial Dilution

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Definition

A method used to stepwise dilute substance into solution with constant dilution factor in each step.

Application

3 Fold Serial Dilution Test

Experimental sciences include biochemistry, pharmacology, and physics as well as in homeopathy.

Difficulty

Since the dilution-fold is the same in each step, the dilutions are a geometric series (constant ratio between any adjacent dilutions). For example: 1/ 3, 1/9, 1/27, 1/81 Notice that each dilution is three - fold relative to the previous one. The first step in making a serial dilution is to take a known volume (usually 1ml) of stock and place it into a known volume of distilled water (usually 9ml). This produces 10ml of the dilute solution. As another example, a 2-fold dilution is the same as a dilution factor of 2. Therefore, dilution by any factor X is equivalent to X-fold dilution. Please note that the dilution factor calculator below uses the metric unit for volume (fL, pL, nL, μL, mL, and L). In normal usage it means to dilute to a total volume of four times what you started with. So if you start with 1 L, you dilute until the total is 4 L. After you have calculated the individual dilutions for each tube, multiply the dilutions when using serial dilutions. Serial dilutions are the culmination of a number of diluted tubes used in order to get smaller dilutions. When a sample diluted 1/100 is added to a sample diluted 1/10, the final dilution would be: (1/100) x (1/10) = 1/1000. Not sure what you mean by 'series', but to make a 5-fold dilution, you would take one part of the culture and add 4 parts. So, take 1 ml of culture and add 4 mls medium to give a final of 5 mls. Or take 10 mls and add 40 mls to give 50 mls. These are then 5-fold dilutions. If you want to make serial dilutions that are each 5-fold, then 1 ml + 4.

3 Fold Serial Dilution Numbers

Procedure: Medium

Concept: Easy

Concept

Serial dilution is the stepwise dilution of a substance in solution. No cd patch download. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion.

Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale.

Besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample.

A tenfold dilution for each step is called a logarithmic dilution or log-dilution and a 3.16-fold (100.5-fold) dilution is called a half-logarithmic dilution or half-log dilution.

Materials

3 Fold Serial Dilution Solution

  • Buffer used to dissolve the sample
  • The sample
  • Multiple tubes
  • Pipette or graduated cylinder
  • Stirring rod

3 Fold Serial Dilution Charts

Procedure

  • Shake the solution by hand or use the stirring rod to swirl the solution. Make sure the solution is uniformly mixed.
  • Take half of the solution out to a new tube and add equal amount of buffer into it to dilute the solution concentration to half of the original solution.
  • Take half of the newly made solution to another new tube and add equal amount of buffer into it to dilute the solution concentration to one quarter of the original solution.
  • Continue the process until reaching the desired concentration of the solution.

Analysis

Serial dilution is a process of solution preparation. Therefore, analysis is not necessary.

3 Fold Serial Dilution Chart

References